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一般說明
MISSION pLKO.1-puro 对照载体是一种慢病毒质粒载体。该载体不含 shRNA 插入，在使用 MISSION shRNA 文库克隆的实验中用作阴性对照。 这让人们可以检测转染对基因表达的影响，并解读 shRNA 克隆看到的敲低效应。氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外，通过与相容的包装质粒共转染，可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒。普尔科。1-puro 对照载体以 10μ 提供g 质粒 DNA，溶于 Tris-EDTA (TE) 缓冲液中，浓度为 500 ng/μl。
應用
MISSION® pLKO.1-puro 空载体对照质粒 DNA 已被用于合成和克隆 shRNA（靶向鼠分化簇 36 (CD36)），以生成慢病毒表达载体。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
法律資訊
使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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