SAB5900206

Alpaca Anti-EGFR, recombinant single domain Antibody

recombinant Alpaca (VHH) Nano antibody

• 分類程式碼代碼: 12352203

屬性

• 生物源: alpaca
• 品質等級: 100
• 重組細胞: expressed in (E. coli)
• 共軛: unconjugated
• 抗體表格: (Affinity purified VHH antibody)
• 無性繁殖: EB61, monoclonal
• 化驗: >95% (SDS-PAGE)
• 形狀: lyophilized solid
• 分子量: 14.4 kDa; ~15-18 kDa (SDS PAGE under reducing condition)
• 純化經由: affinity chromatography (purified via Ni-charged resin)
• 物種活性: human
• 濃度: 1 mg/mL
• 技術: ELISA: 1:1000-1:25000; immunofluorescence: 1:200-1:600; immunohistochemistry: 1:200-1:600
• 顏色: colorless
• pH值: ( in PBS, pH 7.4)
• 同型: VHH
• Protein ID登錄號: AAI18666.1
• UniProt登錄號: P00533
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... EGFR(1956)

描述

一般說明

EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors. Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and erbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis. EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2/neu, ErbB-3/HER-3/neu and ErbB-4/HER-4neu. EGFR can heterodimerize with each of the members of this family.

特異性

Human Epidermal Growth Factor Receptor 1 (HER1/EGFR)

免疫原

Recombinant human EGFR (Leu25-Ser645) protein

應用

ELISA, IHC,IF

包裝

50 µg per vial / 100 µg per vial

外觀

Purified VHH protein in buffer containing 25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA

重構

Reconstitute the lyophilized protein with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

儲存和穩定性

Stable for 1 years at 4°C from date of receipt.

其他說明

Storage at 4°C; Do not freeze.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable