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Key Documents

SAB4200766

Sigma-Aldrich

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal

clone NOS-IN, purified from hybridoma cell culture

同義詞:

Anti-Hepatocyte NOS (HEP-NOS), Anti-Inducible NOS (iNOS), Anti-NOS type II, Anti-Peptidyl-cysteine S-nitrosylase NOS2

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

NOS-IN, monoclonal

形狀

buffered aqueous solution

分子量

~130 kDa

物種活性

human, mouse, rat, plant

濃度

~1.0 mg/mL

技術

flow cytometry: suitable
immunoblotting: 2-4 μg/mL using mouse macrophage RAW 264.7 cell line activated with lipopolysaccharide (LPS) and interferon-γ
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

mouse ... Nos2(18126)

一般說明

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the NOS-IN hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse. Inducible-type nitric oxide synthase (NOS) of mouse macrophage origin (iNOS) or macrophage NOS (mNOS) is a type II NOS (NOS2). It is a Ca2+-independent protein, expressed in activated macrophages and some glial cells after stimulation. NOS2 is also present in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts. The iNOS gene is mapped to human chromosome 17q11.2. iNOS is a dimeric flavoprotein with a heme group. It comprises the C-terminal reductase domain and an N-terminal oxygenase domain.

特異性

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal reacts specifically with inducible-type nitric oxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain (bNOS) or with endothelial-type NOS (eNOS). The antibody recognizes iNOS from human, mouse, rat and plant origin.

免疫原

synthetic peptide corresponding to the C terminal region of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS), conjugated to KLH

應用

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal may be used:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • fluorescence-activated cell sorting (FACS)

生化/生理作用

Inducible nitric oxide synthase (iNOS) or NOS2 catalyzes the oxidation of L-arginine to L-citrulline and nitric oxide (NO) via an OH-L-arginine intermediate in the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and oxygen. Elevated levels of iNOS is observed in asthma. A high level of iNOS leads to an increase in NO production which may contribute to cell death and tissue damage. It is also implicated in the pathophysiology of septic shock. Single nucleotide polymorphism in the iNOS gene is indicative of high risk for B and T-cell non-Hodgkin lymphoma. iNOS also elicits macrophage bactericidal functionality.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

儲存和穩定性

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Krystyna Boczoń et al.
Folia histochemica et cytobiologica, 42(4), 209-213 (2005-02-12)
The aim of this study was to demonstrate iNOS mRNA expression in muscular phase of experimental trichinellosis and to localize iNOS protein in T. spiralis-infected muscles using specific anti-iNOS monoclonal antibodies. The expression of iNOS mRNA in skeletal muscles from
Christian Bogdan
Trends in immunology, 36(3), 161-178 (2015-02-18)
Thirty years after the discovery of its production by activated macrophages, our appreciation of the diverse roles of nitric oxide (NO) continues to grow. Recent findings have not only expanded our understanding of the mechanisms controlling the expression of NO
Nitric oxide. No endothelial NO.
S H Snyder
Nature, 377(6546), 196-197 (1995-09-21)
O Bagasra et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(26), 12041-12045 (1995-12-19)
Nitric oxide (NO) has been implicated as a pathogenic mediator in a variety of central nervous system (CNS) disease states, including the animal model of multiple sclerosis (MS) and experimental allergic encephalomyelitis. We have examined post-mortem brain tissues collected from
iNOS (NOS2) at a glance
Lowenstein CJ and Padalko E
Journal of Cell Science, 117(14), 2865-2867 (2004)

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