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Merck
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重要文件

S5394

Sigma-Aldrich

乏脂血清 来源于胎牛

frozen liquid

同義詞:

Fetal Calf Serum, Lipoprotein Deficient Serum

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.61

生物源

bovine fetus

品質等級

形狀

frozen liquid

濃度

≥14 mg protein/mL Biuret

技術

cell culture | mammalian: suitable
cell culture | stem cell: suitable

雜質

<1 ng/mg protein endotoxin
<5 % of normal lipoprotein

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

研究表明,使用脂蛋白缺乏血清条件下培养的人成纤维细胞中甾醇的产生减少。

應用

来自胎牛的脂蛋白缺乏血清已被用作培养基中的补充剂,用于培养中国仓鼠卵巢(CHO)衍生细胞、胸腺叶和人外周血单核细胞(PBMC)。

準備報告

无菌过滤, 0.2 μm
用 Dulbecco′s 磷酸盐缓冲盐水(pH 7.4-7.5)进行透析

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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分析證明 (COA)

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P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Brian T Emmer et al.
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Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
Utsab Subedi et al.
International journal of molecular sciences, 24(3) (2023-02-12)
Neointimal hyperplasia is characterized by a loss of the contractile phenotype of vascular smooth muscle cells (VSMCs). Our group has recently shown that VSMC proliferation and migration are mediated by lysophosphatidic acid (LPA) during restenosis, but the role of autotaxin
Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP)
Shao W and Espenshade PJ
The Journal of Biological Chemistry, 289(11), 7547-7557 (2014)

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