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Key Documents

S1826

Sigma-Aldrich

唾液酸醛缩酶 来源于大肠杆菌 K12

recombinant, expressed in E. coli BL21, ≥3.0 units/mg protein

同義詞:

N-乙酰神经氨酸丙酮酸裂解酶(N-乙酰基-D-甘露糖胺形成), N-乙酰神经氨酸裂解酶

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About This Item

酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

重組細胞

expressed in E. coli BL21

品質等級

形狀

lyophilized powder

比活性

≥3.0 units/mg protein

分子量

33.4 kDa

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

唾液酸醛缩酶,或N-乙酰神经氨酸裂解酶,可催化N-乙酰神经氨酸的可逆醛醇裂解以形成丙酮酸和N-乙酰基-D-甘露糖胺。N-乙酰神经氨酸裂解酶在自然状态下主要存在于病原体中。

應用

唾液酸醛缩酶可用于合成C(6)至C(10)的非天然糖,以设计糖酶的拮抗剂和抑制剂。

單位定義

在37°C、pH 8.0条件下,一单位将会在每分钟内催化从Man-N-Ac和丙酮酸形成1.0 μmol Neu-5-Ac。

外觀

含有Tris-HCl和NaCl的冻干粉

分析報告

酶活性测定在含有Neu-5-Ac (10 mM) 的Tris-HCl缓冲液(100 mM,pH 7.5)中于37 °C进行15分钟,并使用带有紫外检测器的毛细管电泳在200 nm进行分析。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Chien-Yu Chou et al.
The Journal of biological chemistry, 286(16), 14057-14064 (2011-01-29)
In a recent directed-evolution study, Escherichia coli D-sialic acid aldolase was converted by introducing eight point mutations into a new enzyme with relaxed specificity, denoted RS-aldolase (also known formerly as L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase), which showed a preferred selectivity toward
Guiomar Sánchez-Carrón et al.
Applied and environmental microbiology, 77(7), 2471-2478 (2011-02-15)
N-Acetylneuraminate lyases (NALs) or sialic acid aldolases catalyze the reversible aldol cleavage of N-acetylneuraminic acid (Neu5Ac) to form pyruvate and N-acetyl-d-mannosamine (ManNAc). In nature, N-acetylneuraminate lyase occurs mainly in pathogens. However, this paper describes how an N-acetylneuraminate lyase was cloned
Jozef Nahálka et al.
Organic & biomolecular chemistry, 7(9), 1778-1780 (2009-07-11)
Active inclusion bodies of polyphosphate kinase 3 and cytidine 5'-monophosphate kinase were combined with whole cells that co-express sialic acid aldolase and CMP-sialic acid synthetase. The biocatalytic mixture was used for the synthesis of CMP-sialic acid, which was then converted

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