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Merck
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Key Documents

RAB0352

Sigma-Aldrich

Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr202/pTyr204), phospho-JNK (pThr183/pTyr185), and phospho-p38 MAPK (pThr180/pTyr182) in cultured cell lines

192 assays

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About This Item

分類程式碼代碼:
41116158
NACRES:
NA.32

用途

192 assays

物種活性

mouse, human, rat

技術

ELISA: suitable
capture ELISA: suitable

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

The Cell-Based ERK1/2 (activated) ELISA Sampler Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185) and p38 MAPK (pThr180m / pTyr182) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.

免疫原

ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185), p38 MAPK (pThr180 / pTyr182) synthetic phosphopeptides

應用

请参考Protocol了解详情。

套裝中的組件也可單獨購買

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描述
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  • RABPLATE1Uncoated MicroplateSDS

  • RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDS

  • RABSTOP1Stop SolutionSDS

  • RABTMB1TMB Substrate Reagent (Item J)SDS

  • RABWASH120X Wash Buffer Concentrate ASDS

  • RABWASH220X Wash Buffer Concentrate BSDS

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訊號詞

Danger

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


分析證明 (COA)

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Xiaoqing Zhang et al.
Stem cell research, 32, 115-125 (2018-10-03)
Vascular smooth muscle cells (VSMCs) play essential roles in regulating blood vessel form and function and they are required for vascular tissue regeneration. Multipotent adipose derived stromal cells (ASCs) can be differentiated into VSMC-like cells, which can be used as

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