推薦產品
生物源
bovine
品質等級
化驗
≥96% (agarose gel electrophoresis)
≥96%
形狀
lyophilized powder
分子量
~66 kDa
純化經由
cold ethanol fractionation
包裝
poly bottle of
來源
USA origin
free fatty acid content
≤0.01%
技術
microbiological culture: suitable
雜質
≤0.01% fatty acid (GC)
pH值
7
溶解度
water: soluble (50 mg/ml)
UniProt登錄號
應用
hematology
異物活動
BT virus, none detected
VSV virus, none detected
儲存溫度
2-8°C
基因資訊
bovine ... ALB(280717)
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一般說明
BSA是已知的脂肪酸(FA)载体。因此,控制用于培养细胞系的特定FA′非常重要,因为不同细胞系对特定的脂肪酸可能有不同的敏感性。因此,不含脂肪酸的BSA是细胞培养实验的有用工具,培养实验中必须严格控制特定脂肪酸的含量,从而使研究人员能够使用对其细胞系有特异性的特定脂肪酸。不含脂肪酸的白蛋白还为细胞培养中特定脂肪酸的添加提供了最佳和最大结合位点。使用不含FA的BSA还可以缓解人们对非不含FA的BSA中可能含有内源FA′的担忧。
牛血清白蛋白(BSA)是一种含量丰富的血浆蛋白,有助于调节血液的胶体渗透压。BSA可用于多种不同应用,例如细胞培养、免疫化学和蛋白质定量。
该BSA产品经过专门测试,具有非常低的FA含量/无脂肪酸状态。
该BSA产品经过专门测试,具有非常低的FA含量/无脂肪酸状态。
生化/生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
特點和優勢
- 冷乙醇分级
- 基本上不含脂肪酸
準備報告
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
通常称为第五组分(Cohn fraction V);本品采用改进的Cohn氏冷乙醇分级分离法制备。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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