R9778

Anti-hnRNP-A1 antibody, Mouse monoclonal

~2 mg/mL, clone 4B10, purified from hybridoma cell culture

• 同義詞: Anti-Heterogeneous Nuclear Ribonucleoprotein-A1
• MDL號碼: MFCD06798702
• 分類程式碼代碼: 12352203
• NACRES: NA.41
• 共軛: unconjugated
• application: ARR; ELISA (i); ICC; IP; WB
• 無性繁殖: 4B10, monoclonal
• 物種活性: bovine, human, canine
• citations: 11
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of HeLa cells

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: 4B10, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 32-35 kDa
• 物種活性: bovine, human, canine
• 濃度: ~2 mg/mL
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of HeLa cells
• 同型: IgG2a
• UniProt登錄號: P09651
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... HNRNPA1(3178)

描述

一般說明

Monoclonal Anti-hnRNP-A1 (mouse IgG2a isotype) is derived from the 4B10 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from NZB mice immunized with purified human hnRNPA1. Heterogeneous nuclear ribonucleoprotein A1 (hnRNPs) consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steady-state proteins of the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range of molecular weight starting with 34 kDa up to 43 kDa. hnRNP-A1 is ubiquitously expressed in cells and tissues.

免疫原

purified human hnRNP-A1.

應用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-hnRNP-A1 antibody produced in mouse has also been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry.

生化／生理作用

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP-A1) is important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation and turnover. hnRNP-A1 is important in pre-mRNA processing and in mRNA export from the nucleus. The protein contains a 38-amino acid domain called M9, which is important for the interaction with the transportin protein and therefore, for its import and export from the nucleus. RanGTP mediates dissociation of hnRNP-A1 from transportin. Higher expression is observed in proliferating and/or transformed cells than in differentiated tissues.

外觀

Solution in 0.01 M phosohate buffered saline, pH 7.4, and 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)