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重要文件

PF0100

Sigma-Aldrich

蛋白酶检测试剂盒(荧光法)

high sensitivity assay

同義詞:

蛋白酶活性分析试剂盒

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.26

品質等級

運輸包裝

dry ice

儲存溫度

−20°C

應用

蛋白酶荧光检测试剂盒已用于:
  • 通过基于酪蛋白的蛋白酶测定,检测蛋白水解活性
  • 检测真菌菌株的细胞外蛋白水解活性
  • 定量线粒体蛋白酶活性
  • 测定土壤溶液中的蛋白酶生成

適合性

检测限:最大50ng胰蛋白酶

數量

提供了足以用于200次一毫升反应体系的试剂。
提供了足以用于200次一毫升反应体系的试剂。

三氯乙酸(TCA)组分以6.1 N TCA 溶液的形式提供。0.6 N TC A溶液可通过将所需体积的 T0699 用超纯水进行10倍稀释而制备。使用试剂盒时无需将所有体积的 T0699 均进行稀释。

分析報告

已测试过其在全部4种蛋白酶水解类别中的检测效果(丝氨酸、天冬氨酸、半胱氨酸和金属蛋白酶)。

僅套裝組件

產品號碼
描述

  • Incubation buffer 25 mL

  • FITC-casein solution 5 mL

  • Assay buffer 200 mL

  • Protease standard (trypsin) 20 μg

  • FITC control 50 mg

  • Trichloroacetic acid (TCA) solution 30 mL

訊號詞

Danger

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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條款

The Protease Fluorescent Detection Kit provides ready-to-use reagents for detecting the presence of protease activity. This simple assay to detect protease activity uses casein labeled with fluorescein isothiocyanate (FITC) as the substrate.

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