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Key Documents

P5267

Sigma-Aldrich

L -脯氨酸对硝基苯胺 三氟乙酸盐

prolyl aminopeptidase substrate

同義詞:

N -(4-硝基苯基)吡咯烷-2-甲酰胺, P-pNA, Pro-pNA

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About This Item

經驗公式(希爾表示法):
C11H13N3O3 · C2HF3O2
CAS號碼:
分子量::
349.26
MDL號碼:
分類程式碼代碼:
12352209
eCl@ss:
32160406
PubChem物質ID:
NACRES:
NA.26

品質等級

化驗

≥99% (TLC)

形狀

powder

技術

ligand binding assay: suitable

顏色

white to yellow

儲存溫度

2-8°C

SMILES 字串

OC(=O)C(F)(F)F.[O-][N+](=O)c1ccc(NC(=O)[C@@H]2CCCN2)cc1

InChI

1S/C11H13N3O3.C2HF3O2/c15-11(10-2-1-7-12-10)13-8-3-5-9(6-4-8)14(16)17;3-2(4,5)1(6)7/h3-6,10,12H,1-2,7H2,(H,13,15);(H,6,7)/t10-;/m0./s1

InChI 密鑰

KYRVEVYREUUAKH-PPHPATTJSA-N

一般說明

脯氨酸对硝基苯胺 (p-pNA) 是脯氨酰氨肽酶(脯氨酸亚氨基肽酶)的比色底物,脯氨酸亚氨基肽酶是一种从小肽 N -末端释放脯氨酸的酶。

應用

L-脯氨酸对硝基苯胺三氟乙酸盐还用作单肽底物,测定纤化肽催化剂PC4的氨基分解活性。
脯氨酸对硝基苯胺(p-pNA)已用作卷心菜来源脯氨酰氨肽酶(脯氨酸亚氨基肽酶)的底物。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Kazuyuki Hiwatashi et al.
Bioscience, biotechnology, and biochemistry, 68(6), 1395-1397 (2004-06-25)
We have found a novel prolyl aminopeptidase in Grifola frondosa. The enzyme was purified by DEAE-Sepharose CL-6B, Butyl-Toyopearl, Sephacryl S-100, and Mono-Q column chromatographies. The purified enzyme exists as a dimer and gives high activity toward L-proline-p-nitroanilide. The enzyme was
Paul J Lijnen et al.
Journal of the renin-angiotensin-aldosterone system : JRAAS, 6(2), 69-77 (2006-02-14)
To determine whether the aminopeptidase B inhibitor, arphamenine A, could affect collagen production and expression in control and TGF-ss1-treated cardiac fibroblasts. Cardiac fibroblasts from passage 2 from normal male adult rats were cultured to confluency and incubated with and without
Margarita Marinova et al.
Protein and peptide letters, 16(2), 207-212 (2009-02-10)
Chick-pea (Cicer arietinum L.) cotyledons are unique source of aminopeptidase - 8-9 U/g cotyledons was observed using L-leucine-p-nitroanilide as substrate. The aminopeptidase was purified (65 kDa, pI 4.8 ) reaching a specific activity of 220 U/mg at pH 7.0-7.2 and
Hongyu Yang et al.
World journal of microbiology & biotechnology, 32(11), 176-176 (2016-09-16)
Prolyl aminopeptidases are specific exopeptidases that catalyze the hydrolysis of the N-terminus proline residue of peptides and proteins. In the present study, the prolyl aminopeptidase gene (pap) from Aspergillus oryzae JN-412 was optimized through the codon usage of Pichia pastoris.
Cathal S Mahon et al.
Microbiology (Reading, England), 155(Pt 11), 3673-3682 (2009-06-27)
Fungi are capable of degrading proteins in their environment by secreting peptidases. However, the link between extracellular digestion and intracellular proteolysis has scarcely been investigated. Mycelial lysates of the filamentous fungus Talaromyces emersonii were screened for intracellular peptidase production. Five

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