推薦產品
重組細胞
expressed in CHO cells
品質等級
描述
contains a C-terminal 6-His tag
化驗
≥90% (SDS-PAGE)
形狀
solution
enzyme activity
≥15 units/mg protein
分子量
~61 kDa by SDS-PAGE (reducing)
包裝
vial of 6-12 μg
雜質
≤1.0 EU/μg endotoxin
NCBI登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... NT5E(4907)
一般說明
5′-核苷酸酶是广泛存在于哺乳动物细胞中的胞外酶。有助于从 5′ 加速磷酸基的水解反应核苷酸，产生相应的核苷。
生化／生理作用
5′-核苷酸酶具有多种临床意义。在体外肿瘤血管生成和体内肿瘤免疫逃逸中，它是调控肿瘤细胞增殖、迁移和侵袭的关键分子。由于这一重要角色，该酶是癌症研究的潜在靶点。也参与细胞外核苷酸的补救，并在组织稳态的控制中起关键作用。
5′-核苷酸酶 (nucleotidase) 又称 CD73，是一种能转化核苷-5′−单磷酸生成核苷，底物偏好 AMP。天然 5'-核苷酸酶是一种 GPI 锚定蛋白，其暴露量因缺氧而上调。5′-核苷酸酶在体内有许多功能 ，包括产生细胞外腺苷。
單位定義
一个单元将-37℃、pH 9.0 时每分钟从单磷酸盐水解 1.0μmol腺苷 5′-无机磷。
外觀
提供含 Tris、NaCl、CaCl 2、 和 20% 甘油的溶液。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
F W Sunderman
Annals of clinical and laboratory science, 20(2), 123-139 (1990-03-01)
This review delineates the subcellular distribution, biochemical characteristics, and metabolic functions of 5'-nucleotidase (5'NT), summarizes the analytical biochemistry of 5'NT, and assesses the clinical significance of 5'NT determinations in body fluids, cells, and tissues. Salient aspects of the clinical biochemistry
Zhao-wei Gao et al.
BioMed research international, 2014, 460654-460654 (2014-08-16)
Purinergic signaling has emerged as an important player in cancer progression and is regulated by a series of nucleotidases. Among the enzyme cascade, CD73, which catelyzes AMP breakdown to adenosine, has been found to be overexpressed in many types of
5'-Nucleotidase: molecular structure and functional aspects.
H Zimmermann
The Biochemical journal, 285 ( Pt 2), 345-365 (1992-07-15)
Isolation of a plasma-membrane subfraction from rat liver containing an insulin-sensitive cyclic-AMP phosphodiesterase.
P D House et al.
European journal of biochemistry, 24(3), 429-437 (1972-01-21)
Anna Wilk et al.
Scientific reports, 10(1), 651-651 (2020-01-22)
Changes in nicotinamide adenine dinucleotide (NAD+) levels that compromise mitochondrial function trigger release of DNA damaging reactive oxygen species. NAD+ levels also affect DNA repair capacity as NAD+ is a substrate for PARP-enzymes (mono/poly-ADP-ribosylation) and sirtuins (deacetylation). The ecto-5'-nucleotidase CD73
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