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Merck
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重要文件

MAK532

Sigma-Aldrich

Nitric Oxide Synthase (NOS) Assay Kit

sufficient for 100 colorimetric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

應用

pharmaceutical

檢測方法

colorimetric

相關疾病

cardiovascular diseases; immunological diseases; aging/geriatric diseases; cancer

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules.

特點和優勢

  • Linear detection range: 0.25 - 25 U/L

適合性

The kit is suitable for the determination of nitric oxide synthase activity in biological samples, as well as for studying the effects of drugs on nitric oxide synthase activity.

原則

The Nitric Oxide Synthase (NOS) Assay Kit nvolves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 40 minutes.

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 3


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