MAK512

Malate Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

• 分類程式碼代碼: 12352204
• NACRES: NA.84

屬性

• 品質等級: 100
• 檢測方法: colorimetric
• 相關疾病: Alzheimer′s disease; neurological disorders; neurodegenerative disorders
• 運輸包裝: wet ice
• 儲存溫度: −20°C

描述

一般說明

Malate dehydrogenase (MDH) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 is found in the cytoplasm and plays a key part in the malateaspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer′s disease.

特點和優勢

• Linear detection range: 0.5 to 65 U/L
• Compatible with high-throughput handling systems.

適合性

The kit is suitable for malate dehydrogenase activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)

原則

The Malate Dehydrogenase Assay Kit is a non-radioactive, colorimetric MDH assay based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

安全資訊

• 象形圖: GHS02,GHS05,GHS07
• 訊號詞: Danger
• 危險聲明: H225,H290,H315,H319,H336
• 防範說明: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• 危險分類: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• 標靶器官: Central nervous system
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 3