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Merck
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重要文件

MAK464

Sigma-Aldrich

Lactate Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

同義詞:

LDH Assay Kit, Lactate Dehydrogenase Activity Assay Kit

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.84

品質等級

應用

pharmaceutical

檢測方法

colorimetric

相關疾病

cancer; hematological disorder

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage.

特點和優勢

  • Linear detection range 2-200 U/L
  • Compatible with high-throughput handling systems.
  • Simple procedure allows for reliable quantitation of LDH activity within 30 minutes.

適合性

The kit is suitable for lactate dehydrogenase activity determination in serum, plasma, and other sources. It is also suitable for the characterization and quality control of LDH production, as well as for the screening and evaluation of LDH modulators in drug discovery.

原則

The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity.

訊號詞

Danger

危險分類

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 2


分析證明 (COA)

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