推薦產品
檢測方法
colorimetric
相關疾病
endocrinological disorders, diabetes; cancer
儲存溫度
−20°C
基因資訊
human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)
一般說明
柠檬酸合酶(CS;EC 2.3.3.1)是一种线粒体酶,存在于所有能够进行氧化代谢的细胞中。它在Krebs 三羧酸循环中,催化草酰乙酸合成柠檬酸盐。1,2柠檬酸合酶参与能量产生、脂肪生成和胆固醇生成,其活性遵循昼夜节律模式。
應用
柠檬酸合酶活性检测试剂盒已用于测量样品中柠檬酸合酶的活性。
適合性
适于测定不同生物样品内的柠檬酸合成酶活性,如细胞和组织培养上清液以及纯化所得的线粒体。
原則
柠檬酸合酶活性检测试剂盒提供了一种简单直接的方法,用于测量各种组织、细胞培养物(粘附或悬浮)和纯化线粒体中柠檬酸合酶的活性。使用偶联酶反应测定柠檬酸合酶活性,其产生与存在的酶活性成比例的比色(412 nm)产物。一个单位的柠檬酸合酶是在25 °C和pH 7.2 下,每分钟产生 1.0 mmole CoA的酶的量。
僅套裝組件
產品號碼
描述
- CS Assay Buffer
- CS Substrate Mix
- CS Developer
- GSH Standard, Reduced
- CS Positive Control
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
從最近期的版本中選擇一個:
分析證明 (COA)
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