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Merck
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Key Documents

MAK434

Sigma-Aldrich

Acetaldehyde Assay Kit

Sufficient for 100 colorimetric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

輸入

cell culture medium
tissue
serum
urine
food(s)
beverage(s)
plasma

應用

agriculture
cosmetics
environmental
food and beverages

檢測方法

colorimetric

相關疾病

gastrointestinal diseases

儲存溫度

−20°C

一般說明

Acetaldehyde (CH₃CHO) is a commonly found aldehyde in nature and widely used in various industries. It is a metabolic byproduct of ethanol in the liver and is toxic to the human body. However, it is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. Individuals deficient in aldehyde dehydrogenase experience accumulation of acetaldehyde upon alcohol consumption, leading to facial and body flushing known as alcohol flush reaction or “Asian flush syndrome.” Acetaldehyde buildup has also been linked to alcohol-related hangover effects. Despite its classification as a carcinogen, acetaldehyde is naturally present in many foods and beverages such as ripe fruit, coffee, and wine.

應用

The Acetaldehyde Assay Kit may be used for:
  • Alcohol Metabolism Research
  • Hepatology Research
  • Neurological Research

特點和優勢

  • Simplified Process: Enjoy a hassle-free procedure that requires the addition of a single working reagent and only a 30 minute assay room temperature reaction, saving you time and effort.
  • Convenient and Sensitive: Uses only 20 µL of sample for the determination of acetaldehyde with a detection range of 2 µM to 2mM.
  • High-Throughput Compatibility: Seamlessly integrate our kit into high-throughput handling systems, ensuring efficiency and accuracy.

適合性

The kit is suitable for acetaldehyde determination in biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice).

原則

The Acetaldehyde Assay Kit is based on the aldehyde dehydrogenase catalyzed oxidation of acetaldehyde with the reduction of NAD to NADH. The formed NADH subsequently reduces MTT, producing a colored formazan compound. The intensity of the end product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample.

其他說明

For additional information on our range of Biochemicals, please complete this form.

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

12 - Non Combustible Liquids


分析證明 (COA)

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