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Merck
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重要文件

MAK321

Sigma-Aldrich

Acetaldehyde Assay Kit

sufficient for 100 fluorometric tests

同義詞:

Acetaldehyde Quantitation Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 100 fluorometric tests

輸入

culture(s)
food(s)
serum
plasma
tissue
urine
beverage(s)

應用

cosmetics
food and beverages

檢測方法

fluorometric

相關疾病

gastrointestinal diseases

儲存溫度

−20°C

一般說明

Acetaldehyde is one of the most widely occurring aldehydes in nature and commonly used in industry. The metabolic byproduct of ethanol in the liver, acetaldehyde is toxic to the human body and rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and this accumulation results in facial and body flushing known as alcohol flush reaction. Build up of acetaldehyde has also been associated with the effects of hangovers from alcohol consumption. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine.

應用

The Acetaldehyde Assay Kit may be used for:
  • Food and Beverage Testing
  • Gastrointestinal Disease Research

特點和優勢

Enhanced Detection Range: Detect Acetaldehyde levels accurately across a wide range, from 0.5 µM to 60 µM, using only 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 30 minute room temperature reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

適合性

Suitable for biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice)

原則

The fluorimetric acetaldehyde assay is based on aldehyde dehydrogenase catalyzed oxidation of acetaldehyde, in which the generated NADH reduces a probe making it fluorescent. The fluorescence intensity of the product measured at λex/em = 530/585 nm is directly proportional to acetaldehyde concentration in the sample.

其他說明

For additional information on our range of Biochemicals, please complete this form.

象形圖

Corrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

儲存類別代碼

8A - Combustible corrosive hazardous materials


分析證明 (COA)

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Helmut K Seitz et al.
Genes & nutrition, 5(2), 121-128 (2009-10-23)
Chronic ethanol consumption is a strong risk factor for the development of certain types of cancer including those of the upper aerodigestive tract, the liver, the large intestine and the female breast. Multiple mechanisms are involved in alcohol-mediated carcinogenesis. Among
Mikko Salaspuro
Journal of digestive diseases, 12(2), 51-59 (2011-03-16)
Aldehyde dehydrogenase (ALDH2) and alcohol dehydrogenase (ADH) gene polymorphisms associating with enhanced acetaldehyde exposure and markedly increased cancer risk in alcohol drinkers provide undisputable evidence for acetaldehyde being a local carcinogen not only in esophageal but also in gastric cancer.

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