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Key Documents

MAK013

Sigma-Aldrich

葡萄糖和蔗糖检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

同義詞:

葡萄糖和蔗糖比色/荧光分析试剂盒

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

應用

cosmetics
food and beverages

檢測方法

colorimetric
fluorometric

相關疾病

endocrinological disorders, diabetes

儲存溫度

−20°C

一般說明

蔗糖是一种二糖,蔗糖酶水解葡萄糖和果糖。葡萄糖是细胞呼吸中用于能量产生的主要碳水化合物。当细胞摄取时,葡萄糖能够被糖酵解分解或聚合用于能量储存。

應用

葡萄糖和蔗糖检测试剂盒已被用于:
  • 测定蔗糖、有氧糖酵解和丙酮酸激酶活性
  • 测定草莓中的蛋白质和糖
  • 测定小鼠血浆胰岛素和葡萄糖水平
  • 分析血浆葡萄糖和血浆乳酸并定量测定葡萄糖/蔗糖

適合性

适合于各种生物样本,包括血清,血浆,其他体液,食物和生长因子。

原則

在该检测试剂盒中,葡萄糖被葡萄糖氧化酶氧化,生成的比色(570nm)/荧光 (λex = 535 nm/λem = 587 nm) 产物和葡萄糖含量成正比。为了测量蔗糖,会在反应中加入蔗糖酶,将蔗糖转化成葡萄糖和果糖。总葡萄糖减去自由葡萄糖就是蔗糖的浓度。该试剂盒检测线性范围,葡萄糖荧光分析是0.2-1.0nmole,葡萄糖比色分析是2-10nmole。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Aquatic Chronic 3 - Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

閃點(°F)

188.6 °F - closed cup

閃點(°C)

87 °C - closed cup


分析證明 (COA)

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