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Merck
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重要文件

MAK122

Sigma-Aldrich

磷脂测定试剂盒

sufficient for 100 colorimetric or fluorometric tests

同義詞:

磷脂定量试剂盒

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

應用

cosmetics
food and beverages
pharmaceutical

檢測方法

colorimetric
fluorometric

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

磷脂是一种脂质,是构成细胞膜的重要组成部分,在信号转导中起着重要作用。大多数磷脂包含一分子甘油二酯,一分子磷酸基和一分子胆碱。

在该检测中,磷脂(如卵磷脂、溶血卵磷脂和鞘磷脂)会被酶促水解为胆碱,随后使用胆碱氧化酶和H2O2特异性染料进行测定。反应会产生比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物,此产物与样品中的磷脂浓度成正比。比色法的线性检测范围是3 - 200 μM,荧光法则为0.6 - 20 μM。

應用

磷脂测定试剂盒已用于生化分析 和磷脂定量。

特點和優勢

兼容高通量的操作系统。

適合性

适用于生物样品中的磷脂检测。

原則

在该检测中,磷脂(如卵磷脂、溶血卵磷脂和鞘磷脂)会被酶法水解以释放胆碱,而胆碱则可通过胆碱氧化酶和H2O2-特异性染料所测定。所得的比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物与样品中的磷脂浓度成正比。对于比色测定,检测的线性范围是3 - 200 μM;对于荧光测定,检测的线性范围是0.6 - 20 μM。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Aquatic Chronic 3 - Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids


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