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Key Documents

M7292

Sigma-Aldrich

M16 培养基

With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

同義詞:

Cell Culture Medium, Cell Culture Medium M16, M16 Medium Solution

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

無菌

sterile-filtered

形狀

liquid

技術

cell culture | embryo: suitable

雜質

endotoxin, tested

成分

NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)

運輸包裝

ambient

儲存溫度

2-8°C

應用

M2 和 M16 培养基是植入前期胚胎体外培养的常用培养基。它是一种改良的 Krebs-Ringer 碳酸氢盐溶液,与 Whitten′s 培养基非常相似。M16 含有丙酮酸和乳酸作为能量来源,因为植入前胚胎不能有效利用葡萄糖。

品質

溶液中的沉淀不影响本品性能。

重構

添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。

分析報告

该产品经过测试,能够支持单细胞小鼠胚胎发育成扩大的胚泡。对胚泡发育的最低要求是 80%。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Qian-Qian Sha et al.
Nature communications, 9(1), 3477-3477 (2018-08-30)
Trimethylation of histone H3 on lysine-4 (H3K4me3) is associated with gene-regulatory elements, but its transcription-independent function in cell division is unclear. CxxC-finger protein-1 (CFP1) is a major mediator of H3K4 trimethylation in mouse oocytes. Here we report that oocyte-specific knockout
Xiao-Man Liu et al.
Oncotarget, 7(2), 1155-1167 (2015-12-31)
Mitochondrial dynamics change mitochondrial morphological features and numbers as a part of adaptive cellular metabolism, which is vital for most eukaryotic cells and organisms. A disease or even death of an animal can occur if these dynamics are disrupted. Using
Marie-Christine Birling et al.
Scientific reports, 7, 43331-43331 (2017-03-08)
Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even in this model, generating duplication of
Marco Ragusa et al.
RNA biology, 16(9), 1237-1248 (2019-05-29)
Circular RNAs (circRNAs) have a critical role in the control of gene expression. Their function in spermatozoa (SPZ) is unknown to date. Twenty-eight genes, involved in SPZ/testicular and epididymal physiology, were given in circBase database to find which of them
Hong-Bin Liu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6(15), 1900295-1900295 (2019-08-14)
A number of genes involved in zygotic genome activation (ZGA) have been identified, but the RNA-binding maternal factors that are directly related to ZGA in mice remain unclear. The present study shows that maternal deletion of Igf  2bp2 (also commonly known

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利用 CRISPR-Cas9 基因组编辑技术创建转基因小鼠

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

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