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Merck
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重要文件

M8410

Sigma-Aldrich

矿物油

light oil, (neat)

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

無菌

non-sterile

形狀

light oil

儲存條件

protect from light

折射率

n20/D 1.467 (lit.)

密度

0.84 g/mL at 25 °C (lit.)

形式

neat

InChI 密鑰

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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應用

矿物油已用作微滴胚胎培养中的覆盖层。

注意

该产品并不无菌。矿物油难以灭菌。它只能通过仔细过滤温热的油来灭菌。假定抗生素将会被加入到细胞培养基中。否则,应使用货号M5310,因为它是经无菌过滤的。

分析報告

该材料在微滴胚胎培养物中作为覆盖物已经过测试。(所有经检测的胚胎材料都是使用小鼠胚胎进行检测的。)通常使用BCF1 x BDF1杂合体。该油主要用于防止培养基蒸发,特别是在进行胚胎操作时。1相应的步骤如下:单细胞(BCF1 X BDF1)胚胎在三重复的补充含有0.4% BSA的经胚胎检测的HTF(人输卵管液)培养基微滴中进行培养。如需验证检测,至少70%的单细胞阶段对照胚胎必须在96小时发育至胚泡期。如需产品发布,至少80%的单细胞阶段检测胚胎利用待测材料必须在96小时发育至胚泡期。

同類產品

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

No data available

閃點(°C)

No data available

個人防護裝備

Eyeshields, Gloves


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