推薦產品
生物源
human placenta
品質等級
產品線
BioReagent
形狀
liquid
濃度
0.5 mg/mL in Tris-buffered saline
技術
cell culture | mammalian: suitable
表面覆盖率
1‑2 μg/cm2
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... LAMB1(3912)
應用
层粘连蛋白支持多种细胞类型的生长和分化，包括表皮、内皮、神经、肌肉和肝脏细胞。 建议将其按1-2 μg/cm2用作细胞培养的基质。 最佳的浓度的确取决于细胞类型以及应用或研究目标。
生化／生理作用
层粘连蛋白是动物组织中结构性支架的组成部分。 它们通过内功素和基底膜聚糖与IV型胶原结合，并通过整合素受体、dystroglycan糖蛋白复合物以及Lutheran血型糖蛋白与细胞膜结合。 层粘连蛋白具有胶原结合、细胞粘附、肝素结合和神经突向外生长片段的活性结构域。
成分
层粘连蛋白是一种细胞外基质多结构域三聚体糖蛋白，并且是支持粘附、增殖和分化的基底膜的主要非胶原组分。 层粘连蛋白是由通过多个二硫键连接的A、B1和B2链组成。使用胃蛋白酶处理和免疫亲和层析，层连蛋白产品从人胎盘纯化获得。 SDS-PAGE在50kDa和130-160kDa得到两条主要条带。
注意
推荐在-70℃储存该产品，它能保持稳定2年。
準備報告
该产品是在含有150 mM NaCl的50 mM Tris-HCl中以0.5 mg/mL的浓度提供。 它被0.2μm过滤。在使用前在2-8°C将该溶液缓慢解冻以避免形成凝胶。 作为一种涂层溶液，在灭菌平衡盐溶液中稀释，涂层结构表面有最小体积，在37℃培育2小时。 在细胞铺板之前用PBS洗涤3次。层粘连蛋白可储存在2-8°C达一个月。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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