推薦產品
product name
白血病抑制因子 人, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution (pH 7.4), suitable for cell culture
重組細胞
expressed in E. coli
品質等級
化驗
≥95% (SDS-PAGE)
形狀
buffered aqueous solution (pH 7.4)
效力
0.1-1.5 ng/mL ED50/EC50
品質
endotoxin tested
技術
cell culture | mammalian: suitable
儲存溫度
2-8°C
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生化／生理作用
白血病抑制因子（LIF）是一种多效性糖蛋白，最初被发现在诱导分化成巨噬细胞时，可抑制小鼠骨髓白血病细胞系M1的增殖。LIF的其他活性后来被陆续发现，人们可能了解其各种同义名，包括DIF、D因子、DIA、DRF、CNDF、HILDA、HSF-III和MLPLI。人LIF通过包含190kDa LIF结合α-链（130kDa，小鼠）和130kDa信号转导β-链（gp130）的受体发挥其作用，其与CNTF、OSM、L-6和IL-11公用同一受体。因此，它是细胞因子受体超家族gp130家族的成员。LIF受体已在几种细胞中得到鉴定，包括单核细胞、肝脏、胎盘和胚胎干细胞。天然LIF是高度糖基化的，研究显示根据来源不同，其分子量为32kDa至62kDa，但是没有糖基化似乎不影响其生物活性。LIF由单个基因编码，在人或小鼠中其分泌为含有180个氨基酸的单链糖蛋白，并具有保守的二硫键。人和小鼠LIF共享78％的序列同源性。人LIF可激活小鼠细胞，但小鼠LIF不能激活人体细胞。
外觀
溶于含有0.02％吐温® 20的磷酸盐缓冲盐水中的溶液（经 0.2 μm过滤）。
分析報告
使用人白血病细胞系TF-1在培养物中对人LIF的增殖活性进行测定。
法律資訊
TWEEN is a registered trademark of Croda International PLC
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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