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Merck
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Key Documents

L4402

Sigma-Aldrich

溶葡球菌酶 来源于溶葡萄球菌

BioUltra, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein

同義詞:

甘氨酰-甘氨酸内肽酶

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bacterial (Staphylococcus sp.)

品質等級

產品線

BioUltra

化驗

≥97% (SDS-PAGE)

形狀

lyophilized powder

比活性

≥2,000 units/mg protein

分子量

25 kDa

成份

Protein, 40-60% biuret

濃度

40—60% protein

技術

cell based assay: suitable

適合性

suitable for cell lysis

抗生素活性譜

Gram-positive bacteria

應用

diagnostic assay manufacturing

作用方式

cell wall synthesis | interferes

儲存溫度

−20°C

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一般說明

包装尺寸基于蛋白质含量
化学结构:肽

生化/生理作用

溶葡萄球菌素是一种分子量约为25 kDa的锌内肽酶。 由于溶葡萄球菌酶能裂解 葡萄球菌 类细胞壁肽聚糖层中的聚甘氨酸交联键,它已被发现可用于细胞裂解,并具有潜在的抗菌治疗作用。
活性最佳pH:约 7.5

單位定義

一个单位在 25°C 和 pH 7.5 条件下,在 6.0 ml 反应混合物中可使金黄色酿脓葡萄球菌细胞悬浮液的浊度(A620)在 10 分钟内由 0.250 降至 0.125。

外觀

含有磷酸钾缓冲盐和氯化钠

準備報告

亲和纯化

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Johnny Gutierrez et al.
Open forum infectious diseases, 6(4), ofz090-ofz090 (2019-04-27)
Staphylococcus aureus (SA) bacteremia often requires a long treatment duration with antibiotics to prevent relapse due to the ability of SA to establish reservoirs of infection in sites such as heart and bone. These metastatic sites of infection cannot be
Shaw R Gargis et al.
Applied and environmental microbiology, 76(20), 6944-6946 (2010-08-24)
Resistance to lysostaphin, a staphylolytic glycylglycine endopeptidase, is due to a FemABX-like immunity protein that inserts serines in place of some glycines in peptidoglycan cross bridges. These modifications inhibit both binding of the recombinant cell wall targeting domain and catalysis
Igor Belyansky et al.
The American surgeon, 77(8), 1025-1031 (2011-09-29)
Mesh and wound infections during hernia repair are predominantly caused by Staphylococcus aureus. Human acellular dermis (HAD) is known to lose its integrity in the face of large bacterial loads. The goal of this study was to determine if lysostaphin
Yuliya Yurko et al.
Surgical innovation, 19(1), 20-26 (2011-07-12)
Naturally occurring antimicrobial peptides are possibly the "next frontier" in infection prevention. Binding them to mesh could reduce the rate of mesh infections. This study identifies an antimicrobial agent capable of significant antibacterial activity when bound to mesh. Lysozyme, human
Lysostaphin and clarithromycin: a promising combination for the eradication of Staphylococcus aureus biofilms.
A Aguinaga et al.
International journal of antimicrobial agents, 37(6), 585-587 (2011-04-19)

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