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文件

L2879

Sigma-Aldrich

Lysozyme 氯化物形式 来源于鸡蛋白

Grade VI, ≥35,000 units/mg protein (E1%/282)

同義詞:

肽聚糖iV-乙酰胞壁酰水解酶, 粘肽N-乙酰胞壁质水解酶, 胞壁质酶

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About This Item

CAS號碼:
9066-59-5
酶委員會編號:
3.2.1.17 (BRENDA, IUBMB)
EC號碼:
232-954-0
MDL號碼:
MFCD00131557
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

chicken egg white

種類

Grade VI

形狀

lyophilized powder

比活性

≥35,000 units/mg protein (E1%/282)

分子量

~14.3 kDa

成份

Protein, ≥80%

技術

cell based assay: suitable

適合性

suitable for cell lysis

應用

cell analysis

儲存溫度

−20°C

相關類別

一般說明

溶菌酶，又称为胞壁质酶，是存在于诸如白细胞之类的吞噬细胞中的细菌裂解酶。溶菌酶的命名是因为它能溶解或水解细菌细胞壁中N-乙酰氨基葡萄糖(NAG)和N-乙酰胞壁酸(NAM)之间的β-1-4-糖苷键。该蛋白质存在于动物、植物、细菌和病毒中。还存在于中性粒细胞颗粒、巨噬细胞中，以及血清、唾液、奶、蜂蜜和鸡蛋白中。溶菌酶分为不同类型，包括c型（鸡型）、g型（鹅型）和i型（无脊椎动物型）。

應用

溶菌酶（氯化物形式，来自鸡蛋白）用于：
  • 作为蛋白混合物组分，研究在死端过滤中酵母细胞对蛋白混合物引起的膜污染的影响
  • 组成浸没系统，研究其对沙门菌污染肉鸡的抑菌或杀菌作用
  • 在黏度试验中测定溶菌酶活性    

生化／生理作用

溶菌酶是巨噬细胞介导宿主反应的指示剂，与白细胞死亡有关，具有高周转率。多种慢性炎症、炎症性肠病、血液病和肾病中都发现血清溶菌酶浓度提高。鸡蛋白中的C型溶菌酶常用作蛋白结构和功能的研究模型。胞壁质酶主要对革兰氏阳性菌具有杀菌活性。

單位定義

一个酶活性单位是指在pH 6.24、25°C条件下，以溶壁微球菌（Micrococcus lysodeikticus）悬浮液作为底物，在2.6 mL的反应混合物中（1 cm光路），每分钟产生0.001的ΔA450所需的酶量。

外觀

含氯化钠和乙酸钠的冻干粉。

準備報告

3×结晶

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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文章

Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

條款

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

溶菌酶的酶学测定（EC 3.2.1.17）

该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。

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