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Key Documents

I9640

Sigma-Aldrich

IgG 来源于牛血清

technical grade, ≥80% (SDS-PAGE), buffered aqueous solution

同義詞:

牛IgG

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

共軛

unconjugated

品質等級

等級

technical grade

化驗

≥80% (SDS-PAGE)

形狀

buffered aqueous solution

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

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一般說明

Bovine immunoglobulin, protein source obtained from immunoglobulin derived from serum, is a naturally occurring bioactive proteins and peptides. Fetal calf or bovine serum IgG can be depleted with protein G affinity chromatography and various concentration of this product can be used in RPMI-1640 medium to produce mouse hybridoma cell line CAy-G (anti-HBs IgG1 mAb producing hybridoma cell). The heat aggregated bovine serum IgG is also useful in down regulating mannose receptor expression. IgG from bovine serum reacts specifically with mouse.

免疫原

IgG is isolated from pooled bovine serum.

應用

IgG from bovine serum is used as a supplement in cell culture.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.2 with 15 mM sodium azide as preservative

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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S Schreiber et al.
Journal of immunology (Baltimore, Md. : 1950), 147(4), 1377-1382 (1991-08-15)
We previously described the presence of an inhibitory protein contained in the 20 to 40% (NH4)2SO4 precipitable fraction of FCS that down-regulates expression of mannose receptors on bone marrow-derived macrophages. We now identify aggregated bovine IgG as the main inhibitory
C Aybay et al.
Journal of immunological methods, 233(1-2), 77-81 (2000-01-29)
Fetal calf serum (FCS) was depleted of its immunoglobulin G (IgG) in a rapid procedure using protein G affinity chromatography. 20 ml of FCS was depleted of its IgG in less than 80 min by applying 5 ml of FCS
Manita Shakya et al.
Endocrinology (2021-03-12)
Pro-opiomelanocortin (POMC) neurons form an integral part of the central melanocortin system regulating food intake and energy expenditure. Genetic and pharmacological studies have revealed that defects in POMC synthesis, processing, and receptor signaling lead to obesity. It is well established
Carlos Mendoza-Palomares et al.
Eukaryotic cell, 7(4), 684-697 (2008-02-19)
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.

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