I1784

Anti-Importin α antibody, Mouse monoclonal

clone IM-75, purified from hybridoma cell culture

• 同義詞: Anti-Imp α, Anti-Qip1, Anti-karyopherin α
• MDL號碼: MFCD06798706
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: IM-75, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~60 kDa
• 物種活性: bovine, human, mouse, rat
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using HeLa cell extract
• 同型: IgG2b
• UniProt登錄號: O00629
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... KPNA4(3840) ; mouse ... Kpna4(16649) ; rat ... Kpna4(361959)

描述

一般說明

Monoclonal Anti-Importin α (mouse IgG2b isotype) is derived from the hybridoma IM-75 produced by the fusion of mouse myeloma cells (NS1 cells), and splenocytes from BALB/c mice, immunized with recombinant human importin α. The Importin α family of proteins is comprised of three subfamilies based on amino acid sequence similarity: SRP1-like subfamily (containing the SRP1, importin α5, α6 and α7), Rch1-like subfamily (containing Rch1, pendulin, importin α2), and α3/Qip1-like subfamily (containing α4).

免疫原

recombinant human importin α.

應用

Anti-Importin α antibody, Mouse monoclonal has been used:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry

Monoclonal Anti-Importin α antibody produced in mouse has been used in western blotting.

生化／生理作用

The gene KPNA4 (karyopherin subunit α 4) encodes a 16kDa protein, also referred to as importin α 3 belonging to the family of karyopherins (designated KPNA1 to KPNA4). It participates in the recognition of NLS (nuclear localization sequence) sites and dock NLS-containing proteins destined for transport into the nuclear pore complex. It binds to translocon and participates in the sorting of INM (inner nuclear membrane)-directed integral membrane proteins. It associates with nucleoporins and functions in cargo-translocation across the nuclear membrane. Each KPNA contains an N-terminal repeat domain called the importin β binding site that binds to KPNB1, which in turn directs each KPNA/cargo complex through the nuclear pore. The encoded shuttling protein also functions in NF (nuclear factor)-κB nuclear translocation.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable