I0783

Monoclonal Anti-ILK antibody produced in mouse

~2 mg/mL, clone 65.1, purified immunoglobulin, buffered aqueous solution

• 同義詞: Anti-Integrin-linked protein kinase
• MDL號碼: MFCD01633545
• 分類程式碼代碼: 12352203
• NACRES: NA.44

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 65.1, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~59 kDa
• 物種活性: rat, human, bovine, monkey, mouse, canine
• 濃度: ~2 mg/mL
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.5-2.0 μg/mL using whole cell extract of Chinese hamster ovary cell line (CHO cells)
• 同型: IgG2b
• UniProt登錄號: Q13418
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ILK(3611) ; mouse ... Ilk(16202) ; rat ... Ilk(170922)

描述

一般說明

Monoclonal Anti-ILK (mouse IgG2b isotype) is derived from the 65.1 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from BALB/c mice immunized with purified mouse ILK recombinant protein. Integrin-linked kinase (ILK) is a ubiquitously expressed 50-59 kDa serine/threonine kinase that has three structurally well-conserved domains. A C-terminal domain contains the kinase catalytic site as well as the binding site for integrin β1 cytoplasmic domain. A N-terminal domain contains four ankyrin repeats (ANK).

免疫原

purified mouse ILK recombinant protein.

應用

Monoclonal Anti-ILK antibody produced in mouse has been used in:

• immunostaining
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry
• western blotting

生化／生理作用

Integrin-linked kinase (ILK) is a serine-threonine kinase that interacts with PINCH and parvin to modulate cell adhesion, growth, differentiation, migration and invasion. This kinase binds to integrin β1, β2 and β3 domains to regulate integrin signalling. ILK functions as a receptor-proximal effector for integrin and growth factor dependent signal transduction. ILK is associated with cell cycle progression and oncogenic transformation. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-extracellular matrix (ECM) interactions and by certain growth factors. Negative regulation of ILK is mediated by two phosphatases: phosphatase and tensin homolog (PTEN), a tumor suppressor lipid phosphatase and ILKAP (ILK associated serine/threonine phosphatase), a protein phosphatase 2C (PP2C) protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.