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Merck
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重要文件

I0408

Sigma-Aldrich

转化酶 来源于面包酵母(酿酒酵母

BioReagent, from Saccharomyces cerevisiae, for proteomics

同義詞:

β-D-呋喃果糖苷水解酶, β-D-呋喃果糖苷酶, 蔗糖酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.32

生物源

Saccharomyces cerevisiae

品質等級

等級

for proteomics

產品線

BioReagent

形狀

lyophilized powder

分子量

60 kDa

濃度

≥0.5 mg/vial protein (E1%/280)

儲存溫度

2-8°C

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應用

Invertase Glycoprotein Standard has been used:
  • to generate N-linked glycan library
  • as a negative control to study the binding of lectins to high mannose structures
  • for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis
The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.
用于生产糖果食品和人造蜂蜜。

生化/生理作用

转化酶将蔗糖水解成葡萄糖和果糖,产生无色产物,与产生有色产物的酸水解不同。

其他說明

Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Proteomic kinetic analysis of blister fluid and serum in a patient with drug-induced toxic epidermal necrolysis. A comparison with skin immunohistochemistry
Paquet P, et al.
Current Drug Safety (2012)
A lectin affinity workflow targeting glycosite-specific, cancer-related carbohydrate structures in trypsin-digested human plasma
Analytical biochemistry (2011)
Glycan characterization of the NIST RM monoclonal antibody using a total analytical solution: From sample preparation to data analysis
Hilliard M, et al.
MAbs (2017)
Rachel Morissette et al.
Bioscience reports, 32(6), 577-586 (2012-09-04)
In eukaryotes, GPI (glycosylphosphatidylinositol) lipid anchoring of proteins is an abundant post-translational modification. The attachment of the GPI anchor is mediated by GPI-T (GPI transamidase), a multimeric, membrane-bound enzyme located in the ER (endoplasmic reticulum). Upon modification, GPI-anchored proteins enter
Nayan J Sarma et al.
Nucleus (Austin, Tex.), 3(6), 508-515 (2012-10-11)
Transcriptional regulation is a complex process that requires the integrated action of many multi-protein complexes. The way in which a living cell coordinates the action of these complexes in time and space is still poorly understood. Recent work has shown

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