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R1153

Sigma-Aldrich

RNase B 糖蛋白标准品 来源于牛胰腺

≥90% (SDS-PAGE), lyophilized powder

同義詞:

核糖核酸酶B 来源于牛胰腺, RNase B

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About This Item

CAS號碼:
9001-99-4
EC號碼:
232-646-6
MDL號碼:
MFCD00132184
分類程式碼代碼:
12352204
NACRES:
NA.32

product name

RNase B 糖蛋白标准品 来源于牛胰腺, Proteomics Grade

等級

Proteomics Grade

品質等級

200

化驗

≥90% (SDS-PAGE)

形狀

lyophilized powder

儲存溫度

−20°C

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI 密鑰

CQOVPNPJLQNMDC-UHFFFAOYSA-N

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相關類別

應用

RNase B是带有PNGase F的优选底物，用于证明使用SDS-PAGE或MALDI-MS进行N联去糖基化。PNGase F的活性通常使用RNase B通过监测去糖基化后在12%凝胶中明显的迁移率变化来测定。蛋白质组学级RNase B在酶消化和随后的纯化后已被用作N-聚糖的来源。它也被用作糖蛋白标准品。

其他說明

牛胰腺核糖核酸酶 B 是一种糖蛋白，仅含有 N-连接聚糖。它是由单个结构域组成的球状蛋白，与作为非糖基化核心形式的核糖核酸酶A（RNase A）在混合物中以较少成分的形式天然存在。RNase B 在 Asn 34 处含有一个糖基化位点，在该位点有 5-9 个甘露糖残基连接到壳二糖核心，即 Man 5-9 GlcNAc 2 。由于 Asn 34 糖基化的异质性，RNase B 以 5 种糖基化变体形式存在，分子量约为 15 kDa。

品質

RNAse B糖蛋白标准品已经过高度纯化，以去除污染的RNaseA。

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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Jens Möller et al.
Scientific reports, 3, 2884-2884 (2013-10-08)
To clear pathogens from host tissues or biomaterial surfaces, phagocytes have to break the adhesive bacteria-substrate interactions. Here we analysed the mechanobiological process that enables macrophages to lift-off and phagocytose surface-bound Escherichia coli (E. coli). In this opsonin-independent process, macrophage
Integrated GlycoProteome Analyzer (I-GPA) for automated identification and quantitation of site-specific N-glycosylation
Park GW, et al.
Scientific Reports, 6, 21175-21175 (2016)
Cytosolic nuclease TREX1 regulates oligosaccharyltransferase activity independent of nuclease activity to suppress immune activation
Hasan M, et al.
Immunity, 43(3), 463-474 (2015)
Fabian Higel et al.
mAbs, 6(4), 894-903 (2014-05-23)
N-glycosylation is a complex post-translational modification with potential effects on the efficacy and safety of therapeutic proteins and known influence on the effector function of biopharmaceutical monoclonal antibodies (mAbs). Comprehensive characterization of N-glycosylation is therefore important in biopharmaceutical development. In
Maroof Hasan et al.
Immunity, 43(3), 463-474 (2015-09-01)
TREX1 is an endoplasmic reticulum (ER)-associated negative regulator of innate immunity. TREX1 mutations are associated with autoimmune and autoinflammatory diseases. Biallelic mutations abrogating DNase activity cause autoimmunity by allowing immunogenic self-DNA to accumulate, but it is unknown how dominant frameshift
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HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins on TSKgel® BioAssist S

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins on Proteomix® SCX-NP5

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

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