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Key Documents

H5505

Sigma-Aldrich

组蛋白 来源于小牛胸腺

Type III-S

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About This Item

CAS號碼:
Beilstein:
1895465
MDL號碼:
分類程式碼代碼:
41106305
NACRES:
NA.51

生物源

bovine (calf) thymus

品質等級

種類

Type III-S

形狀

powder

濃度

≥60% protein (biuret)

技術

activity assay: suitable

溶解度

H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow

儲存溫度

2-8°C

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應用

组蛋白适用于:
  • 作为体外激酶测定的阳性对照,检测Cdc2-细胞周期蛋白B1磷酸化GST-CD-4蛋白的能力
  • Cdk5活性测定
  • 组蛋白H1激酶测定
  • 作为聚ADP-核糖(PAR)受体,检测体外PAR聚合酶活性
  • 作为[γ-32P]ATP来源32P的受体,检测小、大黄体细胞来源裂解液的蛋白激酶活性

生化/生理作用

组蛋白是一组DNA结合蛋白,其特征是赖氨酸和精氨酸含量较高。组蛋白的分子量在11-21 kDa之间,取决于具体级分。分离和表征了五种不同的组蛋白级分。H1富含赖氨酸、H2a和H2b略微富含赖氨酸、H3和H4富含精氨酸。H1用于连接染色质链上的各个“念珠”(nu bodies)。

特點和優勢

不要将Sigma“类型”名称与Luck及其同事使用的“分级”名称混淆。

其他說明

经SDS-PAGE鉴定,主要为f1亚型

準備報告

富含赖氨酸的级分,分离和描述依据文献(de Nooij, E. and Westenbrink, H.G., BiochimBiophys.Acta, 62, 608 (1962))。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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