GE28-9187-80

MBPTrap^™ HP

Cytiva 28-9187-80, pack of 5 × 5 mL

• 同義詞: MBP Trap
• 分類程式碼代碼: 23151817
• NACRES: NA.56

屬性

• ligand: dextrin
• 包裝: pack of 5 × 5 mL
• 製造商／商標名: Cytiva 28-9187-80
• 儲存條件: (20% Ehtanol)
• 參數: <20 mL/min flow rate; 42 psi
• 床尺寸: 16 mm × 25 mm
• 床體積: 5 mL
• 柱內徑: 16 mm
• 基質: highly cross-linked 6% agarose
• 樣品平均尺寸: 34 μm
• cleaning: 2-13
• 工作範圍: >7
• 容量: ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)
• 儲存溫度: 2-8°C

描述

一般說明

MBPTrap^™ HP columns prepacked with Dextrin Sepharose^™ High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP). This robust and stable Sepharose High Performance based resin enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.

Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

特點和優勢

• HiTrap^® columns prepacked with Dextrin Sepharose^™ High Performance medium.
• Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
• Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
• Physiological conditions and mild elution preserve the activity of the target protein.
• Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva

MBPTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全資訊

• 象形圖: GHS02,GHS02
• 訊號詞: WarningDanger
• 危險聲明: H226 - H225
• 防範說明: P210 - P235 - P241 - P280 - P210 - P233 - P240 - P241 - P242 - P243 - P303 + P361 + P353 - P501
• 危險分類: Flam. Liq. 2
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 1