GE27-0361-01

Ready-to-go^™ T4 DNA Ligase

Cytiva 27-0361-01, sufficient for 50 reactions

• 同義詞: T4 DNA ligase enzyme
• 分類程式碼代碼: 12352204
• NACRES: NA.53

屬性

• 用途: sufficient for 50 reactions
• 製造商／商標名: Cytiva 27-0361-01

描述

一般說明

Ready-To-Go^™ T4 DNA Ligase. Catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction. Ligation of blunt-ended DNA is greatly stimulated by polyethylene glycol.

應用

Applications include self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA. Each predispensed, single-reaction, ambient-temperature-stable tube contains a glassified reaction mix which, when reconstituted to a final reaction volume of 20 μL, yields a solution of reaction buffer, T4 DNA ligase, and ATP.

特點和優勢

• For covalently-linking DNA fragments in as little as 30 min.
• Single-dose, ambient-temperature-stable reaction mixes.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Ready-To-Go is a trademark of Cytiva

安全資訊

• 儲存類別代碼: 13 - Non Combustible Solids