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GE17-0998-01

Heparin Sepharose 6 Fast Flow

Cytiva 17-0998-01, pack of 50 mL

同義詞:

Heparin Sepharose 6 Fast Flow

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

porcine heparin

包裝

pack of 50 mL

製造商/商標名

Cytiva 17-0998-01

基質

6% cross-linked agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning in place

4-13

工作範圍

4-12

適合性

suitable for bioprocess medium

一般說明

Heparin Sepharose 6 Fast Flow is a well established BioProcess affinity medium designed for capture or intermediate purification steps. This medium is extensively used for industrial purification of several marketed plasma proteins.

Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand.

This medium allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Additional applications include DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.

Heparin Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Heparin Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

特點和優勢

  • Industrial purification of antithrombin III, coagulation factors and other plasma proteins
  • Coupling chemistry gives chemically-stable ligand attachment. Stability of medium solely dictated by heparin ligand.
  • BioProcess medium supported for industrial applications and well-established in approved processes

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol, 0.05 M Sodium Acetate)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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文章

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

條款

DNA binding proteins form an extremely diverse class of proteins sharing a single characteristic, their ability to bind to DNA. Functionally the group can be divided into those responsible for the replication and orientation of the DNA such as histones, nucleosomes and replicases and those involved in transcription such as RNA/DNA polymerases, transcriptional activators and repressors and restriction enzymes.

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

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