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G7017

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于罗曼蜗牛

Type HP-2, aqueous solution, ≥100,000 units/mL

同義詞:

β-葡萄糖醛酸苷酶

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About This Item

CAS號碼:
9001-45-0
酶委員會編號:
3.2.1.31 (BRENDA, IUBMB)
MDL號碼:
MFCD00130629
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Helix pomatia

品質等級

300

種類

Type HP-2

形狀

aqueous solution

比活性

≥100,000 units/mL

secondary activity

≤7,500 units/mL sulfatase

運輸包裝

wet ice

儲存溫度

2-8°C

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相關類別

一般說明

β-来自玛瑙螺的H-2型葡糖醛酸糖苷酶是从罗马蜗牛的消化液中提取的酶的粗溶液。β-来自软体动物的葡糖醛酸糖苷酶也有硫酸酯酶活性。两种活性都可用于代谢物的水解，用于毒理学和生物标志物分析。如需更广泛地了解我们的β-葡糖醛酸糖苷酶产品线及其用途，请参阅我们有关药物代谢物分析的页面

應用

  • 通过消化与葡萄糖醛酸苷结合的化合物而制备的LC / MS样品，用于生物标志物和毒理学研究。
  • 去除葡萄糖醛酸苷结合物，例如用于制备黄芩素。
比较不同酶性能的新技术文章
了解更多
关于Sigma R&D产生的最新应用数据，以优化不同来源的酶对不同药物类别的水解条件，并使用色谱纯化酶降低酯酶活性的影响，从而将6-MAM转化为吗啡

生化／生理作用

内源性β-葡糖醛酸糖苷酶是一种溶酶体酶，参与了葡萄糖醛酸苷修饰的分解代谢产物的消化。
在微生物群中发现的微生物β-葡糖醛酸糖苷酶可以通过水解异种生物质和内生代谢物的葡萄糖醛酸苷结合物而引起代谢物的重吸收。
在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解

特點和優勢

  • 最适pH 4.0 - 5.0
  • 硫酸酯酶最适pH 6.2
  • 硫酸酯酶活性低（<7,500单位/ mL）。
  • 硫酸铵悬浮液。
  • 来自软体动物的β-葡糖醛酸糖苷酶。
  • 可用于低pH样品中葡萄糖醛酸苷的酸水解。

品質

来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。

聯結

与G0876相似，只是由Sigma生产的。

單位定義

在pH 5.0、温度37°C条件下，一单元sigma或改良的Fishman单元1小时会从酚酞葡糖苷酸释放 1.0 μg酚酞（30分钟实验）。
硫酸酯酶单元定义：一单位的硫酸酯酶每小时，pH5.0，37℃能够水解1.0 μmole p-硝基儿茶酚硫酸盐。

外觀

在~1.0 M硫酸铵中含有3 mM叠氮化钠作为保存剂的水相溶液。

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基底

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SRE0037

β-葡萄糖醛酸酶, aqueous solution, β-glucuronidase ≥100,000 units/mL

BGALA-RO

β-葡萄糖醛酸酶/芳基硫酸酯酶, from Helix pomatia

SRE0093

β-葡萄糖醛酸酶 来源于帽贝 (Patella vulgata), aqueous solution

SRE0095

快速β-葡萄糖醛酸酶，重组, from limpets (Patella Vulgata), aqueous solution

SAE0157

Potassium Acetate Buffer Solution, 100 mM, pH 5.0, 0.2 μM filtered

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H317,H334

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

分析證明 (COA)

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W Dennis Ulrey et al.
Journal of AOAC International, 96(4), 917-924 (2013-09-05)
A multilaboratory study of AOAC Official Method 2011.23 was performed to satisfy requirements for Final Action status through the AOAC expert review panel process. The study included nine collaborating laboratories from the United States, Canada, Brazil, and The Netherlands. Five
B De Roos et al.
Journal of internal medicine, 244(6), 451-460 (1999-01-20)
To determine the absorption and urinary excretion of the cholesterol-raising coffee diterpenes cafestol and kahweol in humans. Nine healthy ileostomists consumed a dose of one, two or three cups of French-press coffee together with a standardized breakfast on three separate
Jack R T Darby et al.
The Journal of physiology, 597(20), 5063-5077 (2019-09-05)
Substrate restriction during critical developmental windows of gestation programmes offspring for a predisposition towards cardiovascular disease in adult life. This study aimed to determine the effect of maternal resveratrol (RSV) treatment in an animal model in which chronic fetal catheterisation
Jie Xing et al.
Journal of pharmaceutical and biomedical analysis, 39(3-4), 593-600 (2005-05-17)
The stability of baicalin in buffered aqueous solutions at different pHs and in biological fluids, including plasma, urine and tissue homogenates, were investigated in vitro. Structures of the degradation products of baicalin were elucidated by liquid chromatography-electrospray ion trap mass
Madalina Neacsu et al.
Molecular nutrition & food research, 61(3) (2016-07-01)
Phytophenols present in cereals are metabolised to compounds that could be partly responsible for the reduced risk of chronic diseases and all-cause mortality associated with fibre-rich diets. The bioavailability, form and in vivo concentrations of these metabolites require to be
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Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

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Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver. This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver. It is the responsibility of trained Analytical Services laboratory personnel to follow this procedure as written.

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