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G1512

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于罗曼蜗牛

Type H-5, lyophilized powder, ≥400,000 units/g solid

同義詞:

β-葡萄糖醛酸苷酶

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About This Item

CAS號碼:
9001-45-0
酶委員會編號:
3.2.1.31 (BRENDA, IUBMB)
EC號碼:
232-606-8
MDL號碼:
MFCD00130629
分類程式碼代碼:
12352204
NACRES:
NA.54

種類

Type H-5

品質等級

200

形狀

lyophilized powder

比活性

≥400,000 units/g solid

secondary activity

≤40,000 units/g solid sulfatase

儲存溫度

−20°C

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相關類別

一般說明

来自 玛瑙螺 的 H-2 型β-葡萄糖醛酸酶是一种源自罗马蜗牛的酶粗溶液。许多来源于软体动物的 β-葡糖醛酸酶也含有硫酸酯酶活性。

應用

β-葡萄糖醛酸酶用于水解结合的葡糖苷酸和硫酸盐代谢物。
玛瑙螺 中的β-葡萄糖醛酸苷酶已被用于：
  • 在用于液相色谱-电喷雾离子化-串联质谱 (LC-ESI-MS/MS) 分析的尿样的酶处理
  • 高效液相色谱 (HPLC) 分析的猪或大鼠组织中的槲皮素提取程序中
  • 气相色谱-质谱分析 (GC-MS) 的尿液和血浆样品制备中的酶解作用步骤

比较不同酶性能的新技术文章
了解更多
关于Sigma R&D产生的最新应用数据，以优化不同来源的酶对不同药物类别的水解条件，并使用色谱纯化酶降低酯酶活性的影响，从而将6-MAM转化为吗啡

生化／生理作用

β-葡萄糖醛酸酶（β-GIc）是一种外切糖苷酶，催化复合碳水化合物的分解。在人体内可将结合胆红素转化为非结合形式，使胆红素适于重吸收。
在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解

品質

来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。

聯結

凝胶渗透色谱法进一步纯化 G0876。

單位定義

在pH 5.0、温度37°C条件下，一单元sigma或改良的Fishman单元1小时会从酚酞葡糖苷酸释放 1.0 μg酚酞（30分钟实验）。
硫酸酯酶单元定义：一单位的硫酸酯酶每小时，pH5.0，37℃能够水解1.0 μmole p-硝基儿茶酚硫酸盐。

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H317,H334

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
SLCH1463
SLBV9086
SLBQ4217V
SLBB5858V
049K3778

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Denis M Callewaert et al.
Methods in molecular biology (Clifton, N.J.), 610, 435-449 (2009-12-17)
Isoprostanes are a group of prostaglandin-like compounds that are derived in vivo primarily by free radical-mediated peroxidation of arachidonic acid within phospholipids. The resulting isoprostane moieties are rapidly hydrolyzed, metabolized, and excreted. It is now well recognized that isoprostane levels
Measurement of intact sulfate and glucuronide phytoestrogen conjugates in human urine using isotope dilution liquid chromatography-tandem mass spectrometry with [13C3] isoflavone internal standards
Clarke DB, et al.
Analytical Biochemistry, 309(1), 158-172 (2002)
Tissue distribution of quercetin in rats and pigs
de Boer VCJ, et al.
The Journal of Nutrition, 135(7), 1718-1725 (2005)
GC-MS methods for metabolic profiling of microbial fermentation products of dietary polyphenols in human and in vitro intervention studies
Grun CH, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 871(2), 212-219 (2008)
Maaike M Appeldoorn et al.
The Journal of nutrition, 139(8), 1469-1473 (2009-06-06)
Intervention studies with procyanidin (PC)-rich extracts and products such as cocoa and wine suggest protective effects of PC against cardiovascular diseases. However, there is no consensus on the absorption and metabolism of PC dimers. Interestingly, nothing is known about the
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文章

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

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Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver. This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver. It is the responsibility of trained Analytical Services laboratory personnel to follow this procedure as written.

Using UHPLC/MS (TOF) for Detection of Drugs and Metabolites in Urine Following Optimized Enzymatic Hydrolysis Conditions

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

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