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重組細胞
expressed in E. coli
品質等級
共軛
(Glucosaminoglycan)
無菌
aseptically filled
形狀
buffered aqueous solution
比活性
≥140 units/mg protein
分子量
32 kDa
儲存溫度
2-8°C
應用
内-β-半乳糖苷酶已用于分级蛋白质分离。它可用于人内皮细胞内皮分泌组的糖蛋白质组中的去糖基化。
生化／生理作用
未分枝的重复聚乙酰乳糖胺中的内部β(1-4)半乳糖连接[GlcNAc β(1-3)Gal β (1-4)] 结构是优选的底物。
诸如硫酸角质素的硫酸化结构也会被裂解。底物的支化或岩藻糖基化可减少或完全抑制裂解。半乳糖上C-6的硫酸化将阻断裂解。取决于与优选底物的偏差，新乳酸基团的低聚糖会以极低的速率而被裂解。例如，Gal β(1-3)GlcNAc β(1-3) Gal β(1-4)Glc会以硫酸角质素5X10-5的速率而被裂解
β-半乳糖苷酶可将乳糖切割成单糖成分、葡萄糖和半乳糖。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物异乳糖。
诸如硫酸角质素的硫酸化结构也会被裂解。底物的支化或岩藻糖基化可减少或完全抑制裂解。半乳糖上C-6的硫酸化将阻断裂解。取决于与优选底物的偏差，新乳酸基团的低聚糖会以极低的速率而被裂解。例如，Gal β(1-3)GlcNAc β(1-3) Gal β(1-4)Glc会以硫酸角质素5X10-5的速率而被裂解
β-半乳糖苷酶可将乳糖切割成单糖成分、葡萄糖和半乳糖。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物异乳糖。
單位定義
在pH 5.8、37 °C条件下，一单位酶每分钟可从牛角质角蛋白硫酸盐中释放1.0 μ摩尔的还原性糖。
外觀
20 mM Tris-HCl，pH 7.5中的无菌填充溶液
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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