推薦產品
重組細胞
expressed in baculovirus infected Sf9 cells
品質等級
產品線
PRECISIO® Kinase
化驗
≥70% (SDS-PAGE)
形狀
buffered aqueous glycerol solution
比活性
143-193 nmol/min·mg
分子量
~48 kDa
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... GSK3B(2932)
一般說明
研究领域：细胞信号传导。GSK3B（糖原合成酶激酶3β）是GSK3酶的两种同工异构体之一，两者由不同的基因编码。该蛋白主要存在于细胞质中，但细胞核和线粒体中也有发现。它是一种蛋白质-丝氨酸激酶。该蛋白核心含有一个蛋白激酶结构域，由482个氨基酸组成，分子量为46,712Da。它在组织中表达范围广，在脑组织中表达量最高。
應用
His标记的人类活性GSK3已被用作tau蛋白体外超磷酸化的孵育介质。
生化／生理作用
GSK3是一种丝氨酸-苏氨酸蛋白激酶，最初被确定为磷酸化和抑制糖原合成酶的激酶。GSK3普遍存在于人体组织中，并参与多种生理过程的调节，包括胰岛素对糖原和蛋白质合成的控制以及转录因子AP-1和CREB的调节。人类GSK3瞬时转染到稳定转染了各人类tau异构体的中国仓鼠卵巢细胞中，导致tau蛋白在所有被磷酸化依赖性抗tau抗体检测的位点上过度磷酸化。
糖原合成酶激酶 3β（GSK3β）通过在 Thr-286 上将其磷酸化而负责快速的细胞周期蛋白 D1 周转。在胚胎发育和成体组织细胞增殖过程中作为 Wnt 信号的负调节因子发挥作用。这种蛋白质磷酸化和调节多种蛋白质，如糖原合成酶、胰岛素受体底物-1、轴蛋白和 APC（腺瘤性结肠息肉病）。这种蛋白也被证明与多种与阿尔茨海默病 (AD) 相关的蛋白相互作用。
外觀
50 mM 磷酸氢二钠 pH 7.0、300 mm NaCl、150 mm 咪唑、0.1 mM PMSF、0.2 mm DTT、25% 甘油中提供。
法律資訊
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
C Sutherland et al.
The Biochemical journal, 296 ( Pt 1), 15-19 (1993-11-15)
The beta-isoform of glycogen synthase kinase-3 (GSK3 beta) isolated from rabbit skeletal muscle was inactivated 90-95% following incubation with MgATP and either MAP kinase-activated protein kinase-1 (MAPKAP kinase-1, also termed RSK-2) or p70 S6 kinase (p70S6K), and re-activated with protein
B R Sperber et al.
Neuroscience letters, 197(2), 149-153 (1995-09-08)
Hyperphosphorylated tau protein is the major constituent of the paired helical filament (PHF), the major fibrous component of the neurofibrillary lesions of Alzheimer's disease (AD). Hyperphosphorylation of tau is believed to be the critical event that leads to filament assembly.
C A Grimes et al.
Progress in neurobiology, 65(4), 391-426 (2001-08-31)
Glycogen synthase kinase-3beta (GSK3beta) is a fascinating enzyme with an astoundingly diverse number of actions in intracellular signaling systems. GSK3beta activity is regulated by serine (inhibitory) and tyrosine (stimulatory) phosphorylation, by protein complex formation, and by its intracellular localization. GSK3beta
Richard S Jope et al.
Trends in biochemical sciences, 29(2), 95-102 (2004-04-23)
Glycogen synthase kinase-3 (GSK3) is now recognized as a key component of a surprisingly large number of cellular processes and diseases. Several mechanisms play a part in controlling the actions of GSK3, including phosphorylation, protein complex formation, and subcellular distribution.
Katharine A White et al.
The Journal of cell biology, 217(11), 3965-3976 (2018-10-14)
β-Catenin functions as an adherens junction protein for cell-cell adhesion and as a signaling protein. β-catenin function is dependent on its stability, which is regulated by protein-protein interactions that stabilize β-catenin or target it for proteasome-mediated degradation. In this study
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