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Key Documents

MA104

85102918, African green monkey kidney, Epithelial

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About This Item

分類程式碼代碼:
41106514
eCl@ss:
32190102

product name

MA104, from African green monkey kidney, 85102918, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

生物源

African green monkey kidney

增長模式

Adherent

染色體組型

Not specified

形態學

Epithelial

受體

Not specified

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細胞系來源

Monkey African Green kidney

細胞系描述

Established from an African green monkey foetal kidney. The cells are highly susceptible to Simian rotavirus SA11.

Please note: The species of origin of the MA104 cell line has been shown to be different from that claimed by the originators.

Whitaker & Hayward (1985) found the cell line to originate from an African green monkey and not a Rhesus macaque as was originally claimed. Whitaker AM and Hayward CJ (1985). The characterization of three monkey kidney cell lines. Develop. Biol. Standard. Vol 60: 125-131. PMID: 4043530. Abstract: Three monkey kidney cell lines, Vero, GL-V3 and MA-104 were subjected to karyological analysis to determine their chromosomal stability and to confirm their species of origin. Although the lines were shown to be relatively stable throughout all of the passage levels that were tested, the species of origin of one of them was found to be different from that claimed by the originators. This finding was supported by data from isoenzyme studies.

培養基

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 10% FBS / FCS (F2442).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

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