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Key Documents

E5529

Millipore

红色链霉亲和凝胶

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

形狀

suspension

品質等級

儲存期限

1 yr

技術

western blot: suitable

基質

4% agarose

pH值

7.2

容量

~10 μg(biotin per ml of packed gel)

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

EZ View红色链霉亲和素亲和凝胶是由链霉亲和素共价连接到:
溴化氰活化的4%琼脂糖珠。 旨在捕获(下拉)生物素化的目标分子,例如蛋白质、肽、抗体、核酸、凝集素、受体和配体。

應用

当进行小规模的亲和力捕获(例如免疫沉淀)时,亲和基质很难在微量离心管中看到。 意外吸入树脂会导致结果的定量变化。 EZview红色亲和凝胶大大降低了沉淀损失的风险。 EZview树脂的性能与传统的非彩色亲和凝胶一样,但使得用户可以轻松区分沉淀物与上清液。这与更准确的数据相关,因为损失的蛋白质更少。
适用于免疫沉淀、蛋白质印迹和酶分析。

特點和優勢

  • 可见性增加-红色可降低意外吸入的风险
  • 通过减少意外损失提高目标蛋白的回收率
  • 重现性更高-收率更一致

外觀

含有50%甘油和15 ppm Kathon的PBS的1:1 (v/v)悬液。

法律資訊

EZview is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Alicia M Bostwick et al.
Biochemical and biophysical research communications, 528(3), 580-585 (2020-06-09)
Mammalian cells contain genetic information in two compartments, the nucleus and the mitochondria. Mitochondrial gene expression must be coordinated with nuclear gene expression to respond to cellular energetic needs. To gain insight into the coordination between the nucleus and mitochondria
Marion Mussbacher et al.
Scientific reports, 9(1), 15403-15403 (2019-10-30)
Murine 3T3-L1 adipocytes share many similarities with primary fat cells and represent a reliable in vitro model of adipogenesis. The aim of this study was to probe the effect of S-nitrosoglutathione (GSNO) on adipocyte differentiation. Adipogenesis was induced with a
Katarzyna M Zientara-Rytter et al.
Cells, 11(1) (2022-01-12)
Pex11, an abundant peroxisomal membrane protein (PMP), is required for division of peroxisomes and is robustly imported to peroxisomal membranes. We present a comprehensive analysis of how the Pichia pastoris Pex11 is recognized and chaperoned by Pex19, targeted to peroxisome
Ting-Yu Chang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 138, 111485-111485 (2021-03-20)
Aberrant alteration of epigenetic information disturbs chromatin structure and gene function, thereby facilitating cancer development. Several drugs targeting histone deacetylases (HDACs), a group of epigenetic enzymes, have been approved for treating hematologic malignancies in the clinic. However, patients who suffer
Lu Su et al.
The EMBO journal, 38(8) (2019-03-13)
SREBPs are master regulators of lipid homeostasis and undergo sterol-regulated export from ER to Golgi apparatus for processing and activation via COPII-coated vesicles. While COPII recognizes SREBP through its escort protein SCAP, factor(s) specifically promoting SREBP/SCAP loading to the COPII

相關內容

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

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