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Key Documents

D4668

Sigma-Aldrich

葡聚糖 来源于青霉菌

lyophilized powder, 100-250 units/mg protein

同義詞:

1,6-α-D-葡聚糖 6-葡聚糖水解酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

品質等級

比活性

100-250 units/mg protein

成份

Protein, ~25% Lowry

儲存溫度

2-8°C

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相關類別

應用

来自青霉属的葡聚糖酶可用于修饰Alternan。来自青霉属的葡聚糖酶还用于一项研究中,该研究探索了Dex蛋白可能采用任何已知结构都不熟悉的折叠的可能性。

生化/生理作用

该产品为一种水解葡聚糖中α-(1,6)-α-葡糖苷键的内葡聚糖酶。葡聚糖是通过在糖生产过程中微生物污染物从蔗糖中合成的不良化合物。它们会增加流动的粘度,降低工业的回收率。葡聚糖酶已在糖厂用于水解葡聚糖,以提高糖生产的有效性。

單位定義

在pH 6.0,37 ℃条件下以右旋糖酐为底物,一个单位可在每分钟内释放1.0 μmole的异麦芽糖(以麦芽糖进行测量)。

外觀

部分纯化的冻干粉

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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T Pons et al.
Proteins, 31(4), 345-354 (1998-06-17)
The DEX gene encodes an extracellular dextranase (EC 3.2.1.11); this enzyme hydrolyzes the alpha(1,6) glucosidic bond contained in dextran to release small isomaltosaccharides. Sequence analysis has revealed only one homologous sequence, CB-8 protein, from Arthrobacter sp., with 30% sequence identity.
Timothy D Leathers et al.
Biotechnology letters, 31(2), 289-293 (2008-10-22)
Alternan is a unique glucan with a backbone structure of alternating alpha-(1 --> 6) and alpha-(1 --> 3) linkages. Previously, we isolated strains of Penicillium sp. that modify native, high molecular weight alternan in a novel bioconversion process to a
Dextranase in sugar industry: A review
Efrain Rodriguez Jimenez
Sugar Tech, 11(2), 124-134 (2009)
Young-Min Kim et al.
Applied microbiology and biotechnology, 91(2), 329-339 (2011-04-12)
Multiple forms of native and recombinant endo-dextranases (Dexs) of the glycoside hydrolase family (GH) 66 exist. The GH 66 Dex gene from Streptococcus mutans ATCC 25175 (SmDex) was expressed in Escherichia coli. The recombinant full-size (95.4 kDa) SmDex protein was digested
Lin Chen et al.
Journal of microbiology and biotechnology, 19(12), 1506-1513 (2010-01-16)
Alpha-dextranase, which can hydrolyze dextran, is largely used in the sugar industry. However, a thermostable alpha- dextranase is needed to alleviate the viscosity of syrups and clean blocked machines. Thus, to improve the optimal temperature of Lipomyces starkeyi alpha-dextranase expressed

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