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Key Documents

D3035

Sigma-Aldrich

脱氧核糖核酸 来源于人类胎盘

buffered aqueous solution, sexed, female

同義詞:

DNA

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
41106310
NACRES:
NA.52

生物源

human placenta

品質等級

等級

for molecular biology

形狀

buffered aqueous solution

運輸包裝

dry ice

儲存溫度

−20°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI 密鑰

AWBASQCACWFTGD-UHFFFAOYSA-N

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一般說明

人胎盘DNA是从单一女性供体胎盘中分离得到的,但会含有一些母体DNA。已经使用DNA探针杂交确定了性别。

應用

来自人胎盘的脱氧核糖核苷酸已用于:
  • 比较DNA定量方法的效率
  • 在来自膀胱癌细胞系DNA样品的实时定量PCR分析中作为标准品
  • 作为内参预测游离DNA的片段化程度
  • 用于限制性消化分析以鉴定人染色体22q11的低拷贝重复区域
  • 比较不同人类和灵长目动物的70-bp P5外显子序列
  • 计算相对于正常人组织基因的拷贝数
  • 用于PCR反应
用于Southern杂交。

推薦產品

DNA溶液已在4℃,pH 7.5 - 8.0,含1mM EDTA且不含抑菌剂的10 mM Tris中成功保存数月。在低浓度(约25 μg/ml)条件下,DNA倾向于吸附在塑料管表面上。

相關產品

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Joanne S Aveyard et al.
The Journal of molecular diagnostics : JMD, 6(4), 356-365 (2004-10-28)
Many tumors have large homozygous deletions of the CDKN2A locus (encoding p14(ARF) and p16) and of CDKN2B (p15). Our aim was to determine which gene is the major target in bladder cancer. We used quantitative real-time PCR (RTQ-PCR) to determine
J E Collins et al.
Genome research, 7(5), 522-531 (1997-05-01)
A clone map consisting of YACs, cosmids, and fosmids has been constructed covering low copy repeat regions of human chromosome 22q11. A combination of clone restriction digest analysis, single-copy landmark content analysis, HindIII-Sau3AI fingerprinting, and sequencing of PCR products derived
Alain R Thierry et al.
Nucleic acids research, 38(18), 6159-6175 (2010-05-25)
Although circulating DNA (ctDNA) could be an attractive tool for early cancer detection, diagnosis, prognosis, monitoring or prediction of response to therapies, knowledge on its origin, form and rate of release is poor and often contradictory. Here, we describe an
Heather Hoover et al.
Journal of proteome research, 14(9), 3670-3679 (2015-07-08)
Tumor types can be defined cytologically by their regions of chromosomal amplification, which often results in the high expression of both mRNA and proteins of certain genes contained within the amplicon. An important strategy for defining therapeutically relevant targets in
Karsten Nielsen et al.
Forensic science international. Genetics, 2(3), 226-230 (2008-12-17)
Six commercial preparations of human genomic DNA were quantified using five quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and RB1 rt-PCR. All methods measured higher DNA concentrations than

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