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S8015

Sigma-Aldrich

SSC缓冲剂

for Northern and Southern blotting, powder blend

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About This Item

分類程式碼代碼:
41105319
NACRES:
NA.25

等級

for molecular biology

形狀

powder blend

pH值

6.9-7.1 (20-25 °C, 20 ×)

異物活動

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

儲存溫度

room temp

一般說明

SSC (Saline Sodium Citrate) Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization. SSC buffer powder should be reconstituted to the final indicated volume using molecular biology grade water.

應用

Suitable for use in washing buffer for Northern and Southern hybridization procedures.

特點和優勢

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

成分

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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A J Bogers et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 2(3), 160-166 (1988-01-01)
To identify risk factors for hospital mortality after postinfarction left ventricular aneurysmectomy (PILVA), 83 consecutive cases of PILVA were studied in a retrospective analysis. Hospital mortality was 14.4%. Most of the factors correlated with hospital mortality were associated with poor
Aline V Probst et al.
Developmental cell, 19(4), 625-638 (2010-10-19)
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how
Christèle Maison et al.
Nature genetics, 43(3), 220-227 (2011-02-15)
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of

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In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry

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