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重要文件

D2197

Sigma-Aldrich

心肌黄酶 来源于克鲁氏梭状芽孢

recombinant, expressed in E. coli, Animal-component free

同義詞:

心肌黄酶, 硫辛酰胺脱氢酶, 硫辛酰脱氢酶

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About This Item

酶委員會編號:
分類程式碼代碼:
12352204
NACRES:
NA.54

重組細胞

expressed in E. coli

品質等級

形狀

solid

比活性

>=30 units/mg protein (biuret)

儲存溫度

−20°C

一般說明

研究领域:癌症

應用

克氏梭菌心肌黄酶已用于:
  • 鳃细胞乳酸脱氢酶测定。
  • 浸渍在试纸上进行甲酸脱氢酶测定。
  • 在电子受体存在下氧化β-NADH或β-NADPH的各种测定。
  • 苯醌还原过程中生成半醌。
  • 评估培养肌肉细胞中的葡萄糖摄入情况。
  • 乳酸脱氢酶细胞毒性检测。

生化/生理作用

心肌黄酶在电子受体(例如亚甲基蓝或2,6-二氯苯酚吲哚酚)存在下催化β-NADH或β-NADPH的氧化。 心肌黄酶对β-NADH或β-NADPH具有特异性。Straub的猪心脏酶可能具有天然心肌黄酶(β-NADH特异性)以及硫辛酸和硫辛酰胺脱氢酶活性。

單位定義

一个单位的心肌黄酶将在25℃、pH 7.5下每分钟氧化1.0微摩尔的β-NADH,并相应还原适当的电子受体。

外觀

以含有海藻糖冻干粉形式提供。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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