CRISPR20V

Lenti CRISPR Universal Non-Target Control#3 Transduction Particles (LV04 vector)

• 分類程式碼代碼: 41106609
• NACRES: NA.51

屬性

• 形狀: liquid
• 品質等級: 200
• 包裝: vial of 200 μL
• 濃度: (1x10⁶ TU/mL (via p24 titering assay))
• 應用: CRISPR
• 運輸包裝: dry ice
• 儲存溫度: −70°C

描述

一般說明

The Lenti CRISPR Non-Target Control#3 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#3 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Control#3 uses a dual-component system consisting of U6-driven guide RNA (non-target#3) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

應用

功能性基因组学研究/筛选/靶标验证

其他說明

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

法律資訊

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable