OE21 Cell Line human

Caucasian oesophageal squamous cell carcinoma, 96062201

• 同義詞: JROECL 21 Cells, OE 21 Cells
• 分類程式碼代碼: 41106514

屬性

• 生物源: human esophagus
• 增長模式: Adherent
• 染色體組型: Aneuploid
• 形態學: Epithelial
• 產品: Not specified
• 受體: MHC class I
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian oesophageal squamous cell carcinoma

細胞系描述

OE21, also known as JROECL21, was established in 1993 from a squamous carcinoma of mid oesophagus of a 74 year-old male patient. The tumour was identified as pathological stage IIA(UICC) and showed moderate differentiation. HLA-A,-B and -C (MHC class I) are expressed constitutively, expression of ICAM-1 can be induced by treatment with interferon-gamma. OE21 cells express epithelial cytokeratins and are tumourigenic in nude mice.

應用

Study of oesophageal cancer

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 13
D5S818: 12,13
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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