OE33 Cell Line human

96070808, human esophagus, Epithelial

• 同義詞: JROECL33, JROECL33 Cells, OE-33 Cells
• 分類程式碼代碼: 41106514

屬性

• product name: OE33 Cell Line human, 96070808, Caucasian esophageal carcinoma
• 生物源: human esophagus
• 增長模式: Adherent
• 染色體組型: Aneuploid
• 形態學: Epithelial
• 產品: Not specified
• 受體: MHC class I, ICAM-1
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian oesophageal carcinoma

細胞系描述

The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett′s metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.

應用

OE33 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells. It has also been used to study the sensitivity of the cells to anti-c-Met monoclonal antibody ABT-700.

Study of oesophageal cancer

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

例行更新培養

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days (i.e. replacing half the old medium with fresh).

其他說明

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