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TGP 54

94022430, mouse pancreas, Not specified

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About This Item

分類程式碼代碼:
41106514

產品名稱

TGP 54, 94022430

生物源

mouse pancreas

增長模式

Adherent

染色體組型

Not specified

形態學

Not specified

產品

Somatostatin

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mouse pancreatic islet cell tumour

細胞系描述

TGP 54 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E)Bri8 mouse. These mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 54 cells are SV40T antigen positive and secrete low amounts of somatostatin, it is one of a series of cells obtained from pancreatic tumours deposited at ECACC.

應用

Study of exocrine pancreas carcinomas, hormone secretion studies.

培養基

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

其他說明

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