CRI-G5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• 分類程式碼代碼: 41106514

屬性

• 生物源: rat pancreas
• 包裝: tube of 5 μg 88031603-DNA-5UG; pkg of vial of cells 88031603-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Insulin and glucagon
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rat NEDH islet tumour

細胞系描述

Established from a NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon. This cell line can be used in place of CRI-D11 (Sigma Catalogue number. 88031604).

應用

Hormone studies

培養基

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. The cells tend to grow in clumps and ribbons.

其他說明

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