TM4

88111401

• 分類程式碼代碼: 41106514

屬性

• 生物源: mouse testis
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Transferrin, H-Y Ag, novel retinol-binding protein
• 受體: Androgen, oestrogen and progesterone receptors
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse BALB/c testis sertoli cell

細胞系描述

From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.

應用

Hormone studies

培養基

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.

其他說明

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